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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat CD69 ORF mammalian expression plasmid, C-GFPSpark tag||RG80209-ACG|
|Rat CD69 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80209-ACR|
|Rat CD69 ORF mammalian expression plasmid, C-Flag tag||RG80209-CF|
|Rat CD69 ORF mammalian expression plasmid, C-His tag||RG80209-CH|
|Rat CD69 ORF mammalian expression plasmid, C-Myc tag||RG80209-CM|
|Rat CD69 ORF mammalian expression plasmid, C-HA tag||RG80209-CY|
|Rat CD69 ORF mammalian expression plasmid, N-Flag tag||RG80209-NF|
|Rat CD69 ORF mammalian expression plasmid, N-His tag||RG80209-NH|
|Rat CD69 ORF mammalian expression plasmid, N-Myc tag||RG80209-NM|
|Rat CD69 ORF mammalian expression plasmid, N-HA tag||RG80209-NY|
|Rat CD69 natural ORF mammalian expression plasmid||RG80209-UT|
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Early activation antigen CD69, also known as activation inducer molecule (AIM), is a single-pass type II membrane protein. Recently, cDNA clones encoding human and mouse CD69 were isolated and showed CD69 to be a member of the C-type lectin superfamily. It is one of the earliest cell surface antigens expressed by T cells following activation. Once expressed, CD69 acts as a costimulatory molecule for T cell activation and proliferation. In addition to mature T cells, CD69 is inducibly expressed by immature thymocytes, B cells, natural killer (NK) cells, monocytes, neutrophils and eosinophils, and is constitutively expressed by mature thymocytes and platelets. CD69 is involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. The structure, chromosomal localization, expression and function of CD69 suggest that it is likely a pleiotropic immune regulator , potentially important in the activation and differentiation of a wide variety of hematopoietic cells. This membrane molecule transiently expresses on activated lymphocytes, and its selective expression in inflammatory infiltrates suggests that it plays a role in the pathogenesis of inflammatory diseases. CD69 plays a crucial role in the pathogenesis of allergen-induced eosinophilic airway inflammation and hyperresponsiveness and that CD69 could be a possible therapeutic target for asthmatic patients.