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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat IL25 ORF mammalian expression plasmid, C-GFPSpark tag||RG80194-ACG|
|Rat IL25 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80194-ACR|
|Rat IL25 ORF mammalian expression plasmid, C-Flag tag||RG80194-CF|
|Rat IL25 ORF mammalian expression plasmid, C-His tag||RG80194-CH|
|Rat IL25 ORF mammalian expression plasmid, C-Myc tag||RG80194-CM|
|Rat IL25 ORF mammalian expression plasmid, C-HA tag||RG80194-CY|
|Rat IL25 ORF mammalian expression plasmid, N-Flag tag||RG80194-NF|
|Rat IL25 ORF mammalian expression plasmid, N-His tag||RG80194-NH|
|Rat IL25 ORF mammalian expression plasmid, N-Myc tag||RG80194-NM|
|Rat IL25 ORF mammalian expression plasmid, N-HA tag||RG80194-NY|
|Rat IL25 natural ORF mammalian expression plasmid||RG80194-UT|
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Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17–producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP)–activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided.