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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat EPHA4 ORF mammalian expression plasmid, C-GFPSpark tag||RG80123-ACG|
|Rat EPHA4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80123-ACR|
|Rat EPHA4 ORF mammalian expression plasmid, C-Flag tag||RG80123-CF|
|Rat EPHA4 ORF mammalian expression plasmid, C-His tag||RG80123-CH|
|Rat EPHA4 ORF mammalian expression plasmid, C-Myc tag||RG80123-CM|
|Rat EPHA4 ORF mammalian expression plasmid, C-HA tag||RG80123-CY|
|Rat EPHA4 ORF mammalian expression plasmid, N-Flag tag||RG80123-NF|
|Rat EPHA4 ORF mammalian expression plasmid, N-His tag||RG80123-NH|
|Rat EPHA4 ORF mammalian expression plasmid, N-Myc tag||RG80123-NM|
|Rat EPHA4 ORF mammalian expression plasmid, N-HA tag||RG80123-NY|
|Rat EPHA4 natural ORF mammalian expression plasmid||RG80123-UT|
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EPH receptor A4 (ephrin type-A receptor 4), also known as EphA4, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus, and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor−mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). The extracellular domain of an EphA4 interacts with ephrin ligands, which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.