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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat TGFB1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80116-ACG|
|Rat TGFB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80116-ACR|
|Rat TGFB1 ORF mammalian expression plasmid, C-Flag tag||RG80116-CF|
|Rat TGFB1 ORF mammalian expression plasmid, C-His tag||RG80116-CH|
|Rat TGFB1 ORF mammalian expression plasmid, C-Myc tag||RG80116-CM|
|Rat TGFB1 ORF mammalian expression plasmid, C-HA tag||RG80116-CY|
|Rat TGFB1 ORF mammalian expression plasmid, N-Flag tag||RG80116-NF|
|Rat TGFB1 ORF mammalian expression plasmid, N-His tag||RG80116-NH|
|Rat TGFB1 ORF mammalian expression plasmid, N-Myc tag||RG80116-NM|
|Rat TGFB1 ORF mammalian expression plasmid, N-HA tag||RG80116-NY|
|Rat TGFB1 natural ORF mammalian expression plasmid||RG80116-UT|
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TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.