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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat WFDC2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80084-ACG|
|Rat WFDC2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80084-ACR|
|Rat WFDC2 ORF mammalian expression plasmid, C-Flag tag||RG80084-CF|
|Rat WFDC2 ORF mammalian expression plasmid, C-His tag||RG80084-CH|
|Rat WFDC2 ORF mammalian expression plasmid, C-Myc tag||RG80084-CM|
|Rat WFDC2 ORF mammalian expression plasmid, C-HA tag||RG80084-CY|
|Rat WFDC2 ORF mammalian expression plasmid, N-Flag tag||RG80084-NF|
|Rat WFDC2 ORF mammalian expression plasmid, N-His tag||RG80084-NH|
|Rat WFDC2 ORF mammalian expression plasmid, N-Myc tag||RG80084-NM|
|Rat WFDC2 ORF mammalian expression plasmid, N-HA tag||RG80084-NY|
|Rat WFDC2 natural ORF mammalian expression plasmid||RG80084-UT|
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WAP four-disulfide core domain protein 2, also known as Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2 and HE4, is a secreted protein which contains two WAP domains. WFDC2 / HE4 is a member of a family of stable 4-disulfide core proteins that are secreted at high levels. It is expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. It is highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. WFDC2 may be a component of the innate immune defences of the lung, nasal and oral cavities and suggest that WFDC2 functions in concert with related WAP domain containing proteins in epithelial host defence. WFDC2 re-expression in lung carcinomas may prove to be associated with tumour type and should be studied in further detail. Mammary gland expression of tammar WFDC2 during the course of lactation showed WFDC2 was elevated during pregnancy, reduced in early lactation and absent in mid-late lactation. WFDC2 / HE4 can undergo a complex series of alternative splicing events that can potentially yield five distinct WAP domain containing protein isoforms.