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Rat IL9R Gene cDNA clone plasmid

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Rat IL9R cDNA Clone Product Information
Gene_bank_ref_id:NM_017021.1
RefSeq ORF Size:1404bp
cDNA Description:Full length Clone DNA of Rattus norvegicus interleukin 9 receptor.
Gene Synonym:IL9R
Species:Rat
Vector:pMD18-T Simple Vector
Plasmid:pMD-ratIL9R
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for six point mutations: 1084 C/T resulting in the amino acid pro substitution by ser, 1346 T/A resulting in the amino acid leu substitution by gln, 251 G/T, 399 A/C, 1107 T/C and 1314 G/A not causing the amino acid variation.
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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References
  • Demoulin JB. et al. (1998) Interleukin 9 and its receptor: an overview of structure and function. Int Rev Immunol. 16 (3-4): 345-64.
  • Sliva D. et al. (1999) Tip60 interacts with human interleukin-9 receptor alpha-chain. Biochem Biophys Res Commun. 263 (1): 149-55.
  • Renauld JC. et al. (1995) Interleukin-9 and its receptor: involvement in mast cell differentiation and T cell oncogenesis. J Leukoc Biol. 57 (3): 353-60.
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    Catalog: RG80077-M
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