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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Rat INHBA ORF mammalian expression plasmid, C-GFPSpark tag||RG80051-ACG|
|Rat INHBA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80051-ACR|
|Rat INHBA ORF mammalian expression plasmid, C-Flag tag||RG80051-CF|
|Rat INHBA ORF mammalian expression plasmid, C-His tag||RG80051-CH|
|Rat INHBA ORF mammalian expression plasmid, C-Myc tag||RG80051-CM|
|Rat INHBA ORF mammalian expression plasmid, C-HA tag||RG80051-CY|
|Rat INHBA ORF mammalian expression plasmid, N-Flag tag||RG80051-NF|
|Rat INHBA ORF mammalian expression plasmid, N-His tag||RG80051-NH|
|Rat INHBA ORF mammalian expression plasmid, N-Myc tag||RG80051-NM|
|Rat INHBA ORF mammalian expression plasmid, N-HA tag||RG80051-NY|
|Rat INHBA natural ORF mammalian expression plasmid||RG80051-UT|
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Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. The activin and inhibin protein complexes are both dimeric in structure, and, in each complex, the two monomers are linked to one another by a single disulfide bond. Activin is composed of two β subunits, βA βA (activin A), βB βB (activin B), or βA βB (activin AB). Inhibin is composed of an alpha and one of two β subunits, βA (inhibin A) or βB (inhibin B). Activins are produced in many cell types and organs, such as gonads, pituitary gland, and placenta. In the ovarian follicle, activin increases FSH binding and FSH-induced aromatization. It participates in androgen synthesis enhancing LH action in the ovary and testis. In the male, activin enhances spermatogenesis. In addition, Activin plays a role in wound repair and skin morphogenesis. Activin is strongly expressed in wounded skin, and overexpression of activin in epidermis of transgenic mice improves wound healing and enhances scar formation. Activin also regulates the morphogenesis of branching organs such as the prostate, lung, and kidney. There is also evidence showed that lack of activin during development results in neural developmental defects.