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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Rat GHR ORF mammalian expression plasmid, C-GFPSpark tag||RG80029-ACG|
|Rat GHR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80029-ACR|
|Rat GHR ORF mammalian expression plasmid, C-Flag tag||RG80029-CF|
|Rat GHR ORF mammalian expression plasmid, C-His tag||RG80029-CH|
|Rat GHR ORF mammalian expression plasmid, C-Myc tag||RG80029-CM|
|Rat GHR ORF mammalian expression plasmid, C-HA tag||RG80029-CY|
|Rat GHR ORF mammalian expression plasmid, N-Flag tag||RG80029-NF|
|Rat GHR ORF mammalian expression plasmid, N-His tag||RG80029-NH|
|Rat GHR ORF mammalian expression plasmid, N-Myc tag||RG80029-NM|
|Rat GHR ORF mammalian expression plasmid, N-HA tag||RG80029-NY|
|Rat GHR natural ORF mammalian expression plasmid||RG80029-UT|
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Growth hormone receptor, also known as GH receptor and GHR, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and type 1 subfamily. GHR contains one fibronectin type-III domain. Growth hormone receptor / GHR is expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 of GHR is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression of GHR in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 of GHR is expressed in lung, stomach and muscle. Growth hormone receptor / GHR is a receptor for pituitary gland growth hormone. It is involved in regulating postnatal body growth. On ligand binding, it couples to the JAK2 / STAT5 pathway. Isoform 2 of GHR up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling. Defects in GHR are a cause of Laron syndrome (LARS) which is a severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may also be a cause of idiopathic short stature autosomal (ISSA) which is defined by a subnormal rate of growth.