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Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 Gene cDNA clone plasmid

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Mouse SRC cDNA Clone Product Information
Gene_bank_ref_id:NM_009271.3
RefSeq ORF Size:1626bp
cDNA Description:Full length Clone DNA of Mus musculus Rous sarcoma oncogene transcript variant 1.
Gene Synonym:AW259666, pp60c-src, Src
Species:Mouse
Vector:pGEM-T Vector
Plasmid:pGEM-mSRC
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 Gene cDNA clone plasmid on other vectors
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-GFPSpark tagMG51118-ACG$345
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-OFPSpark / RFP tagMG51118-ACR$345
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, N-GFPSpark tagMG51118-ANG$345
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, N-OFPSpark / RFP tagMG51118-ANR$345
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-Flag tagMG51118-CF$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-His tagMG51118-CH$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-Myc tagMG51118-CM$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-HA tagMG51118-CY$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, Flag tagMG51118-G-F$395
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, N-Flag tagMG51118-NF$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, N-His tagMG51118-NH$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, N-Myc tagMG51118-NM$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, N-HA tagMG51118-NY$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 natural ORF mammalian expression plasmidMG51118-UT$315
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Background

Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

References
  • Juan Ma. et al., 2003, Neuroscience Letters. 348 (3): 185-189.
  • Czernilofsky AP. et al., 1980, Nature. 287: 198-203.
  • Beischlag TV. et al., 2002, Molecular and cellular biology. 22 (12): 4319-33.
  • Size / Price
    Catalog: MG51118-G
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"