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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Mouse SHH ORF mammalian expression plasmid, C-GFPSpark tag||MG51100-ACG|
|Mouse SHH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51100-ACR|
|Mouse SHH ORF mammalian expression plasmid, C-Flag tag||MG51100-CF|
|Mouse SHH ORF mammalian expression plasmid, C-His tag||MG51100-CH|
|Mouse SHH ORF mammalian expression plasmid, C-Myc tag||MG51100-CM|
|Mouse SHH ORF mammalian expression plasmid, C-HA tag||MG51100-CY|
|Mouse SHH ORF mammalian expression plasmid, N-Flag tag||MG51100-NF|
|Mouse SHH ORF mammalian expression plasmid, N-His tag||MG51100-NH|
|Mouse SHH ORF mammalian expression plasmid, N-Myc tag||MG51100-NM|
|Mouse SHH ORF mammalian expression plasmid, N-HA tag||MG51100-NY|
|Mouse SHH natural ORF mammalian expression plasmid||MG51100-UT|
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Sonic HedgeHog, also known as sonic hedgehog protein, belongs to the hedgehog family. It cannot be detected in adult tissues while can be found in fetal intestine, liver, lung, and kidney. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5, triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type 3.