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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Mouse GP1bb ORF mammalian expression plasmid, C-GFPSpark tag||MG51075-ACG|
|Mouse GP1bb ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51075-ACR|
|Mouse GP1bb ORF mammalian expression plasmid, C-Flag tag||MG51075-CF|
|Mouse GP1bb ORF mammalian expression plasmid, C-His tag||MG51075-CH|
|Mouse GP1bb ORF mammalian expression plasmid, C-Myc tag||MG51075-CM|
|Mouse GP1bb ORF mammalian expression plasmid, C-HA tag||MG51075-CY|
|Mouse GP1bb ORF mammalian expression plasmid, N-Flag tag||MG51075-NF|
|Mouse GP1bb ORF mammalian expression plasmid, N-His tag||MG51075-NH|
|Mouse GP1bb ORF mammalian expression plasmid, N-Myc tag||MG51075-NM|
|Mouse GP1bb ORF mammalian expression plasmid, N-HA tag||MG51075-NY|
|Mouse GP1bb natural ORF mammalian expression plasmid||MG51075-UT|
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Platelet glycoprotein Ib (GPIb) complex is best known as a major platelet receptor for von Willebrand factor essential for platelet adhesion under high shear conditions found in arteries and in thrombosis. The GPIb complex is composed of GPIb alpha (Platelet glycoprotein Ib alpha chain) covalently attached to GPIb beta (Platelet glycoprotein Ib beta chain) and noncovalently complexed with GPIX and GPV. GPIb-beta, also known as GP1BB, CD42b-beta and CD42c, is single-pass type I membrane protein expressed in heart and brain, which is a critical component of the von Willebrand factor (vWF) receptor. The cysteine knot region of GPIb beta in the N terminus is critical for the conformation of GPIb beta that interacts with GPIX. The precursor of GP1BB is synthesized from a 1.0 kb mRNA expressed in plateletes and megakaryocytes. GPIb is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alpha chain and 22 kD beta chain. GPIb alpha chain provides the vWF binding site, and GPIb beta chain contributes to surface expression of the receptor and participates in transmembrane signaling through phosphorylation of its intracellular domain. GP1BB is part of the GPIb-V-IX system that constitutes the receptor for von Willebrand factor (vWF), and mediates platelet adhesion in the arterial circulation. Defects in GP1BB are a cause of Bernard-Soulier syndrome (BSS), also known as giant platelet disease (GPD). BSS patients have unusually large platelets and have a clinical bleeding tendency.