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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Mouse FLT1 ORF mammalian expression plasmid, C-GFPSpark tag||MG51045-ACG|
|Mouse FLT1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51045-ACR|
|Mouse FLT1 ORF mammalian expression plasmid, C-Flag tag||MG51045-CF|
|Mouse FLT1 ORF mammalian expression plasmid, C-His tag||MG51045-CH|
|Mouse FLT1 ORF mammalian expression plasmid, C-Myc tag||MG51045-CM|
|Mouse FLT1 ORF mammalian expression plasmid, C-HA tag||MG51045-CY|
|Mouse FLT1 ORF mammalian expression plasmid, N-Flag tag||MG51045-NF|
|Mouse FLT1 ORF mammalian expression plasmid, N-His tag||MG51045-NH|
|Mouse FLT1 ORF mammalian expression plasmid, N-Myc tag||MG51045-NM|
|Mouse FLT1 ORF mammalian expression plasmid, N-HA tag||MG51045-NY|
|Mouse FLT1 natural ORF mammalian expression plasmid||MG51045-UT|
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Vascular endothelial growth factor receptor 1, also known as VEGFR-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor and FLT1, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR-1 / FLT1 contains seven Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. VEGFR-1 / FLT1 is expressed mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. It is specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR-1 / FLT1 is not expressed in tumor cell lines. VEGFR-1 / FLT1 is an essential receptor tyrosine kinase that regulates mammalian vascular development and embryogenesis. EGF-induced angiogenesis requires inverse regulation of VEGFR-1 and VEGFR-2 in tumor-associated endothelial cells. VEGFR-1 / FLT1 is a receptor for VEGF, VEGFB and PGF. It has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.