|Datasheet||Specific References||Reviews||Related Products||Protocols|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Mouse STIM1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50955-ACG|
|Mouse STIM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50955-ACR|
|Mouse STIM1 ORF mammalian expression plasmid, C-Flag tag||MG50955-CF|
|Mouse STIM1 ORF mammalian expression plasmid, C-His tag||MG50955-CH|
|Mouse STIM1 ORF mammalian expression plasmid, C-Myc tag||MG50955-CM|
|Mouse STIM1 ORF mammalian expression plasmid, C-HA tag||MG50955-CY|
|Mouse STIM1 ORF mammalian expression plasmid, N-Flag tag||MG50955-NF|
|Mouse STIM1 ORF mammalian expression plasmid, N-His tag||MG50955-NH|
|Mouse STIM1 ORF mammalian expression plasmid, N-Myc tag||MG50955-NM|
|Mouse STIM1 ORF mammalian expression plasmid, N-HA tag||MG50955-NY|
|Mouse STIM1 natural ORF mammalian expression plasmid||MG50955-UT|
|Learn more about expression Vectors|
Stromal interaction molecule 1, also known as STIM1 and GOK, is a cell membrane, a single-pass type I membrane protein and a endoplasmic reticulum membrane protein. STIM1 / GOK is ubiquitously expressed in various human primary cells and tumor cell lines. It contains one EF-hand domain and one SAM (sterile alpha motif) domain. STIM1 / GOK plays a role in mediating Ca2+ influx following depletion of intracellular Ca2+ stores. It acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, STIM1 / GOK translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A / ORAI1. Transfection of STIM1 / GOK into cells derived from a rhabdoid tumor and from a rhabdomyosarcoma that do not express detectable levels of STIM1 can induce cell death, suggesting a possible role in the control of rhabdomyosarcomas and rhabdoid tumors. Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) which is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels.