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Mouse CCDC80 Gene cDNA clone plasmid

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Mouse CCDC80 cDNA Clone Product Information
Gene_bank_ref_id:NM_026439.2
RefSeq ORF Size:2850bp
cDNA Description:Full length Clone DNA of Mus musculus coiled-coil domain containing 80.
Gene Synonym:Urb, DRO1, Ssg1, 2610001E17Rik, Ccdc80
Species:Mouse
Vector:pGEM-T Vector
Plasmid:pGEM-mCCDC80
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for thirteen point mutations: 100 A/G resulting in the amino acid Thr substitution by Ala,344 G/A resulting in the amino acid Arg substitution by His, 888 G/C resulting in the amino acid Glu substitution by Asp, 1330 A/T resulting in the amino acid Thr substitution by Ser, 1366 G/A resulting in the amino acid Ala substitution by Thr,1442 C/T resulting in the amino acid Ala substitution by Val, 1520 A/C resulting in the amino acid Asp substitution by Ala, 1724 C/G resulting in the amino acid Thr substitution by Ser, 300 T/C, 1242 G/A, 1269 A/G, 1965 G/A and 2079 C/T not causing the amino acid variation.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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Catalog: MG50907-G
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