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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Mouse GFRA3 ORF mammalian expression plasmid, C-GFPSpark tag||MG50901-ACG|
|Mouse GFRA3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50901-ACR|
|Mouse GFRA3 ORF mammalian expression plasmid, C-Flag tag||MG50901-CF|
|Mouse GFRA3 ORF mammalian expression plasmid, C-His tag||MG50901-CH|
|Mouse GFRA3 ORF mammalian expression plasmid, C-Myc tag||MG50901-CM|
|Mouse GFRA3 ORF mammalian expression plasmid, C-HA tag||MG50901-CY|
|Mouse GFRA3 ORF mammalian expression plasmid, N-Flag tag||MG50901-NF|
|Mouse GFRA3 ORF mammalian expression plasmid, N-His tag||MG50901-NH|
|Mouse GFRA3 ORF mammalian expression plasmid, N-Myc tag||MG50901-NM|
|Mouse GFRA3 ORF mammalian expression plasmid, N-HA tag||MG50901-NY|
|Mouse GFRA3 natural ORF mammalian expression plasmid||MG50901-UT|
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Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 3 (GFRA3) or GDNFRa3 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA3 / GDNFRa3 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. The neurotrophic growth factor artemin binds selectively to GDNF family receptor α3 (GFRA3 / GDNFRa3), forming a molecular complex with the co-receptor RET which mediates downstream signaling. This signaling pathway has been demonstrated to play an important role in the survival and maintenance of nociceptive sensory neurons and in the development of sympathetic neurons.