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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Mouse GLIPR1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50869-ACG|
|Mouse GLIPR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50869-ACR|
|Mouse GLIPR1 ORF mammalian expression plasmid, C-Flag tag||MG50869-CF|
|Mouse GLIPR1 ORF mammalian expression plasmid, C-His tag||MG50869-CH|
|Mouse GLIPR1 ORF mammalian expression plasmid, C-Myc tag||MG50869-CM|
|Mouse GLIPR1 ORF mammalian expression plasmid, C-HA tag||MG50869-CY|
|Mouse GLIPR1 ORF mammalian expression plasmid, N-Flag tag||MG50869-NF|
|Mouse GLIPR1 ORF mammalian expression plasmid, N-His tag||MG50869-NH|
|Mouse GLIPR1 ORF mammalian expression plasmid, N-Myc tag||MG50869-NM|
|Mouse GLIPR1 ORF mammalian expression plasmid, N-HA tag||MG50869-NY|
|Mouse GLIPR1 natural ORF mammalian expression plasmid||MG50869-UT|
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Glioma pathogenesis-related protein 1, also known as Protein RTVP-1, GLIPR1 and GLIPR, is a single-pass membrane protein which belongs to the CRISP family. GLIPR1 / RTVP-1 was expressed in high levels in glioblastomas, whereas its expression in low-grade astrocytomas and normal brains was very low. Transfection of glioma cells with small interfering RNAs targeting GLIPR1 / RTVP-1 decreased cell proliferation in all the cell lines examined and induced cell apoptosis in some of them. Overexpression of GLIPR1 / RTVP-1 increased astrocyte and glioma cell proliferation and the anchorage-independent growth of the cells. In addition, overexpression of GLIPR1 / RTVP-1 rendered glioma cells more resistant to the apoptotic effect of tumor necrosis factor-related apoptosis-inducing ligand and serum deprivation. GLIPR1 / RTVP-1 regulated the invasion of glioma cells was evident by their enhanced migration through Matrigel and by their increased invasion in a spheroid confrontation assay. The increased invasive potential of the GLIPR1 / RTVP-1 overexpressors was also shown by the increased activity of matrix metalloproteinase 2 in these cells. The expression of GLIPR1 / RTVP-1 is correlated with the degree of malignancy of astrocytic tumors and that GLIPR1 / RTVP-1 is involved in the regulation of the growth, survival, and invasion of glioma cells. GLIPR1 / RTVP-1 is a potential therapeutic target in gliomas.