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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Mouse PTPRC ORF mammalian expression plasmid, C-GFPSpark tag||MG50713-ACG|
|Mouse PTPRC ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50713-ACR|
|Mouse PTPRC ORF mammalian expression plasmid, C-Flag tag||MG50713-CF|
|Mouse PTPRC ORF mammalian expression plasmid, C-His tag||MG50713-CH|
|Mouse PTPRC ORF mammalian expression plasmid, C-Myc tag||MG50713-CM|
|Mouse PTPRC ORF mammalian expression plasmid, C-HA tag||MG50713-CY|
|Mouse PTPRC ORF mammalian expression plasmid, N-Flag tag||MG50713-NF|
|Mouse PTPRC ORF mammalian expression plasmid, N-His tag||MG50713-NH|
|Mouse PTPRC ORF mammalian expression plasmid, N-Myc tag||MG50713-NM|
|Mouse PTPRC ORF mammalian expression plasmid, N-HA tag||MG50713-NY|
|Mouse PTPRC natural ORF mammalian expression plasmid||MG50713-UT|
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The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexs or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.