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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse CD300A ORF mammalian expression plasmid, C-GFPSpark tag||MG50675-ACG|
|Mouse CD300A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50675-ACR|
|Mouse CD300A ORF mammalian expression plasmid, C-Flag tag||MG50675-CF|
|Mouse CD300A ORF mammalian expression plasmid, C-His tag||MG50675-CH|
|Mouse CD300A ORF mammalian expression plasmid, C-Myc tag||MG50675-CM|
|Mouse CD300A ORF mammalian expression plasmid, C-HA tag||MG50675-CY|
|Mouse CD300A ORF mammalian expression plasmid, N-Flag tag||MG50675-NF|
|Mouse CD300A ORF mammalian expression plasmid, N-His tag||MG50675-NH|
|Mouse CD300A ORF mammalian expression plasmid, N-Myc tag||MG50675-NM|
|Mouse CD300A ORF mammalian expression plasmid, N-HA tag||MG50675-NY|
|Mouse CD300A natural ORF mammalian expression plasmid||MG50675-UT|
|Learn more about expression Vectors|
Mouse CMRF35-like molecule 8, also known as CD300 antigen-like family member A, CMRF35-H9, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CD300a and CMRF35H, is a single-pass type I membrane protein which belongs to the CD300 family. The CD300 family of myeloid immunoglobulin receptors includes activating ( CD300b, CD300e ) and inhibitory members ( CD300a, CD300f ), as well as molecules presenting a negative charge within their transmembrane domain ( CD300c, CD300d ). CD300A / IGSF12 is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.It contains one Ig-like V-type ( immunoglobulin-like ) domain. CD300A / IGSF12 is an inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. CD300c is a functional immune receptor able to deliver activating signals upon ligation in RBL-2H3 mast cells. CD300c signaling is partially mediated by a direct association with the immune receptor tyrosine-based activation motif-bearing adaptor FcεRγ. CD300a and CD300c play an important role in the cross-regulation of TNF-alpha and IFN-alpha secretion from plasmacytoid dendritic cells (pDCs).