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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse PLK1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50624-ACG|
|Mouse PLK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50624-ACR|
|Mouse PLK1 ORF mammalian expression plasmid, N-GFPSpark tag||MG50624-ANG|
|Mouse PLK1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50624-ANR|
|Mouse PLK1 ORF mammalian expression plasmid, C-Flag tag||MG50624-CF|
|Mouse PLK1 ORF mammalian expression plasmid, C-His tag||MG50624-CH|
|Mouse PLK1 ORF mammalian expression plasmid, C-Myc tag||MG50624-CM|
|Mouse PLK1 ORF mammalian expression plasmid, C-HA tag||MG50624-CY|
|Mouse PLK1 ORF mammalian expression plasmid, N-Flag tag||MG50624-NF|
|Mouse PLK1 ORF mammalian expression plasmid, N-His tag||MG50624-NH|
|Mouse PLK1 ORF mammalian expression plasmid, N-Myc tag||MG50624-NM|
|Mouse PLK1 ORF mammalian expression plasmid, N-HA tag||MG50624-NY|
|Mouse PLK1 natural ORF mammalian expression plasmid||MG50624-UT|
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Serine / threonine-protein kinase PLK1 / PLK-1, also known as polo-like kinase 1 (PLK-1) or serine / threonine-protein kinase 13 (STPK13), Polo-like kinases (PLKs), is a family of four serine / threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1 / PLK-1 is ubiquitously expressed. The mRNA and protein expression of PLK1 / PLK-1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. PLK1 / PLK-1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 / PLK-1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 / PLK-1 overexpression correlates with cellular proliferation and poor prognosis. Serine / threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC / C inhibitors, and the regulation of mitotic exit and cytokinesis. It is required for recovery after DNA damage checkpoint and entry into mitosis. PLK1 / PLK-1 is required for kinetochore localization of BUB1B, spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. PLK1 / PLK-1 Phosphorylates BORA, and thereby promotes the degradation of BORA. PLK1 / PLK-1 also contributes to the regulation of AURKA function and phosphorylates SGOL1.