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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse ACHE ORF mammalian expression plasmid, C-GFPSpark tag||MG50543-ACG|
|Mouse ACHE ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50543-ACR|
|Mouse ACHE ORF mammalian expression plasmid, C-Flag tag||MG50543-CF|
|Mouse ACHE ORF mammalian expression plasmid, C-His tag||MG50543-CH|
|Mouse ACHE ORF mammalian expression plasmid, C-Myc tag||MG50543-CM|
|Mouse ACHE ORF mammalian expression plasmid, C-HA tag||MG50543-CY|
|Mouse ACHE ORF mammalian expression plasmid, N-Flag tag||MG50543-NF|
|Mouse ACHE ORF mammalian expression plasmid, N-His tag||MG50543-NH|
|Mouse ACHE ORF mammalian expression plasmid, N-Myc tag||MG50543-NM|
|Mouse ACHE ORF mammalian expression plasmid, N-HA tag||MG50543-NY|
|Mouse ACHE natural ORF mammalian expression plasmid||MG50543-UT|
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Acetylcholinesterase, also known as ACHE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. Acetylcholinesterase plays a crucial role in nerve impulse transmission at cholinergic synapses by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE appears to be a potential therapeutic target at muscle injuries including organophosphate myopathy. It is an externally oriented membrane-bound enzyme and its main physiological role is termination of chemical transmission at cholinergic synapses and secretory organs by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE plays important roles in the cholinergic system, and its dysregulation is involved in a variety of human diseases. ACHE was significantly down-regulated in the cancerous tissues of 69.2% of hepatocellular carcinoma (HCC) patients, and the low ACHE expression in HCC was correlated with tumor aggressiveness, an elevated risk of postoperative recurrence, and a low survival rate. Both the recombinant ACHE protein and the enhanced expression of ACHE significantly inhibited HCC cell growth in vitro and tumorigenicity in vivo. ACHE as a tumor growth suppressor in regulating cell proliferation, the relevant signaling pathways, and the drug sensitivity of HCC cells. Thus, ACHE is a promising independent prognostic predictor for HCC recurrence and the survival of HCC patients. ACHE is responsible for the hydrolysis of acetylcholine in the nervous system. It is inhibited by organophosphate and carbamate pesticides. However, this enzyme is only slightly inhibited by organophosphorothionates.