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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse CD2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50537-ACG|
|Mouse CD2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50537-ACR|
|Mouse CD2 ORF mammalian expression plasmid, C-Flag tag||MG50537-CF|
|Mouse CD2 ORF mammalian expression plasmid, C-His tag||MG50537-CH|
|Mouse CD2 ORF mammalian expression plasmid, C-Myc tag||MG50537-CM|
|Mouse CD2 ORF mammalian expression plasmid, C-HA tag||MG50537-CY|
|Mouse CD2 ORF mammalian expression plasmid, N-Flag tag||MG50537-NF|
|Mouse CD2 ORF mammalian expression plasmid, N-His tag||MG50537-NH|
|Mouse CD2 ORF mammalian expression plasmid, N-Myc tag||MG50537-NM|
|Mouse CD2 ORF mammalian expression plasmid, N-HA tag||MG50537-NY|
|Mouse CD2 natural ORF mammalian expression plasmid||MG50537-UT|
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T-cell surface antigen CD2, also known as T-cell surface antigen T11/Leu-5, and SRBC, is a single-pass type I membrane protein. It contains one Ig-like C2-type domain and one Ig-like V-type domain. CD2 is a cell adhesion molecule expressed on T cells and is recognized as a target for CD48 (rats) and CD58 (humans). CD2 has been shown to set quantitative thresholds in T cell activation both in vivo and in vitro. Further, intracellular CD2 signaling pathways and networks are being discovered by the identification of several cytosolic tail binding proteins. CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain of CD2 is implicated in the signaling function. The complex of CD2 and CD58 also plays an important role in enhancing the adhesion of T lymphocytes to target cells, and promoting hyperplasia and activation of T lymphocytes. As a cell surface glycoprotein, CD2 expressed on most human T cells and natural killer (NK) cells and plays an important role in mediating cell adhesion in both T-lymphocytes and in signal transduction.