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Mouse SERPINA3N Gene cDNA clone plasmid

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Mouse Serpina3n cDNA Clone Product Information
Gene_bank_ref_id:NM_009252.2
RefSeq ORF Size:1257bp
cDNA Description:Full length Clone DNA of Mus musculus serine (or cysteine) peptidase inhibitor, clade A, member 3N.
Gene Synonym:Spi2-2, Spi2.2, Spi2/eb.4, Serpina3n
Species:Mouse
Vector:pMD18-T Simple Vector
Plasmid:pMD-mSERPINA3N
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for fourteen point mutations: 37 G/A resulting in the amino acid Trp substitution by STOP, 59 G/C resulting in the amino acid Cys substitution by Ser, 206 A/C resulting in the amino acid Lys substitution by Thr, 250 G/C resulting in the amino acid Val substitution by Leu, 253 A/G resulting in the amino acid Met substitution by Val,467 G/A resulting in the amino acid Arg substitution by Lys, 469 G/A resulting in the amino acid Ala substitution by Thr, 817 A/C resulting in the amino acid Met substitution by Leu, 842 A/G resulting in the amino acid Lys substitution by Arg, 516 T/A, 771 T/G, 813 T/C, 867 G/A, 1086 C/T not causing the amino acid variation.
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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Catalog: MG50518-M
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"