|Datasheet||Specific References||Reviews||Related Products||Protocols|
pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse MERTK ORF mammalian expression plasmid, C-GFPSpark tag||MG50514-ACG|
|Mouse MERTK ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50514-ACR|
|Mouse MERTK ORF mammalian expression plasmid, C-Flag tag||MG50514-CF|
|Mouse MERTK ORF mammalian expression plasmid, C-His tag||MG50514-CH|
|Mouse MERTK ORF mammalian expression plasmid, C-Myc tag||MG50514-CM|
|Mouse MERTK ORF mammalian expression plasmid, C-HA tag||MG50514-CY|
|Mouse MERTK ORF mammalian expression plasmid, N-Flag tag||MG50514-NF|
|Mouse MERTK ORF mammalian expression plasmid, N-His tag||MG50514-NH|
|Mouse MERTK ORF mammalian expression plasmid, N-Myc tag||MG50514-NM|
|Mouse MERTK ORF mammalian expression plasmid, N-HA tag||MG50514-NY|
|Mouse MERTK natural ORF mammalian expression plasmid||MG50514-UT|
|Learn more about expression Vectors|
&Proto-oncogene tyrosine-protein kinase MER (MERTK) is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. MERTK is localized in membrane and is no expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. This protein is highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver. MERTK regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. MERTK plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Defects in MERTK are the cause of retinitis pigmentosa type 38.