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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse CD19 ORF mammalian expression plasmid, C-GFPSpark tag||MG50510-ACG|
|Mouse CD19 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50510-ACR|
|Mouse CD19 ORF mammalian expression plasmid, C-Flag tag||MG50510-CF|
|Mouse CD19 ORF mammalian expression plasmid, C-His tag||MG50510-CH|
|Mouse CD19 ORF mammalian expression plasmid, C-Myc tag||MG50510-CM|
|Mouse CD19 ORF mammalian expression plasmid, C-HA tag||MG50510-CY|
|Mouse CD19 ORF mammalian expression plasmid, N-Flag tag||MG50510-NF|
|Mouse CD19 ORF mammalian expression plasmid, N-His tag||MG50510-NH|
|Mouse CD19 ORF mammalian expression plasmid, N-Myc tag||MG50510-NM|
|Mouse CD19 ORF mammalian expression plasmid, N-HA tag||MG50510-NY|
|Mouse CD19 natural ORF mammalian expression plasmid||MG50510-UT|
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The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 19 (CD19) is a member of CD system. CD19 is a cell surface molecule that assembles with the antigen receptor of B-cells. This results in a descent in threshold for antigen receptor-dependent stimulation. A simplified view holds that the ability of B-cells to respond to the various antigens in a specific and sensitive manner is achieved in the presence of low-affinity antigen receptors. CD19 primarily acts as a B-cell coreceptor in conjunction with CD21 and CD81. The formation of the receptor complex is induced by antigen and CD19, induced by exogenous antigen, has been found cytoplasmic tail phosphorylated and bind to sIg.