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Mouse SIRT3 ORF mammalian expression plasmid, His tag

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Mouse SIRT3 cDNA Clone Product Information
Gene_bank_ref_id:NM_022433.2
RefSeq ORF Size:774bp
cDNA Description:Full length Clone DNA of Mus musculus sirtuin 3 (silent mating type information regulation 2, homolog) 3 (S. cerevisiae) with His tag.
Gene Synonym:Sir2l3, AI848213, 2310003L23Rik, Sirt3
Species:Mouse
Vector:pCMV/hygro-His
Plasmid:pCMV-mSIRT3-10his
Restriction Site:HindIII + XhoI (5.5kb + 0.8kb)
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pCMV/hygro-HIs Vector Information
 
Vector Name pCMV/hygro-His
Vector Size 5687bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag His
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

Schematic of pCMV/hygro-His Multiple Cloning Sites

His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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Catalog: MG50478-M-H
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