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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse EGFL7 ORF mammalian expression plasmid, C-GFPSpark tag||MG50472-ACG|
|Mouse EGFL7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50472-ACR|
|Mouse EGFL7 ORF mammalian expression plasmid, C-Flag tag||MG50472-CF|
|Mouse EGFL7 ORF mammalian expression plasmid, C-His tag||MG50472-CH|
|Mouse EGFL7 ORF mammalian expression plasmid, C-Myc tag||MG50472-CM|
|Mouse EGFL7 ORF mammalian expression plasmid, C-HA tag||MG50472-CY|
|Mouse EGFL7 ORF mammalian expression plasmid, N-Flag tag||MG50472-NF|
|Mouse EGFL7 ORF mammalian expression plasmid, N-His tag||MG50472-NH|
|Mouse EGFL7 ORF mammalian expression plasmid, N-Myc tag||MG50472-NM|
|Mouse EGFL7 ORF mammalian expression plasmid, N-HA tag||MG50472-NY|
|Mouse EGFL7 natural ORF mammalian expression plasmid||MG50472-UT|
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Epidermal growth factor-like protein 7, also known as EGF-like protein 7, Multiple epidermal growth factor-like domains protein 7, Multiple EGF-like domains protein 7, Vascular endothelial statin, NOTCH4-like protein and EGFL7, is a secreted protein which contains two EGF-like domains and one EMI domain. EGFL7 was identified by a number of groups as a putative secreted factor produced by the vascular endothelial cells (ECs). EGFL7 is described as a novel endothelial cell-derived factor involved in the regulation of the spatial arrangement of cells during vascular tube assembly. With its impact on tubulogenesis and vessel shape EGFL7 joined the large family of molecules governing blood vessel formation. EGFL7 regulates midline angioblast migration in zebrafish embryos-a key step in vascular tubulogenesis. EGFL7 is tightly associated with the extracellular matrix (ECM), and it supports EC migration either as a single factor or in combination with other ECM molecules. EGFL7 provides a proper microenvironment for endothelial cell migration, thereby enabling accurate patterning. Our study indicates that the molecular composition of the ECM influences vascular morphogenesis. EGFL7 also regulates vascular tubulogenesis. It inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate.