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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse JAM2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50464-ACG|
|Mouse JAM2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50464-ACR|
|Mouse JAM2 ORF mammalian expression plasmid, C-Flag tag||MG50464-CF|
|Mouse JAM2 ORF mammalian expression plasmid, C-His tag||MG50464-CH|
|Mouse JAM2 ORF mammalian expression plasmid, C-Myc tag||MG50464-CM|
|Mouse JAM2 ORF mammalian expression plasmid, C-HA tag||MG50464-CY|
|Mouse JAM2 ORF mammalian expression plasmid, N-Flag tag||MG50464-NF|
|Mouse JAM2 ORF mammalian expression plasmid, N-His tag||MG50464-NH|
|Mouse JAM2 ORF mammalian expression plasmid, N-Myc tag||MG50464-NM|
|Mouse JAM2 ORF mammalian expression plasmid, N-HA tag||MG50464-NY|
|Mouse JAM2 natural ORF mammalian expression plasmid||MG50464-UT|
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Junctional adhesion molecule B (JAM-B), also known as Junctional adhesion molecule 2 (JAM2), Vascular endothelial junction-associated molecule (VE-JAM), and CD322, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily. It is prominently expressed on high endothelial venules. expression to be restricted to the high endothelial venule of tonsil and lymph nodes. The localization to the endothelium of arterioles in and around inflammatory and tumor foci. JAM-B can function as an adhesive ligand for the T cell line J45 and can interact with GM-CSF/IL-4-derived peripheral blood dendritic cells, circulating CD56(+) NK cells, circulating CD56(+)CD3(+) NK/T cells, and circulating CD56(+)CD3(+)CD8(+) cytolytic T cells. JAM-2 is expressed on high endothelial venules (HEVs) in human tonsil and on a subset of human leukocytes, suggesting that JAM-2 plays a central role in the regulation of transendothelial migration. It binds to very late activation antigen (VLA)-4, a leucocyte integrin that contributes to rolling and firm adhesion of lymphocytes to endothelial cells through binding to vascular cell adhesion molecule (VCAM)-1. JAM-B appears to contribute to leucocyte extravasation by facilitating not only transmigration but also rolling and adhesion. JAM-B acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs.