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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse PDGF-C ORF mammalian expression plasmid, C-GFPSpark tag||MG50458-ACG|
|Mouse PDGF-C ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50458-ACR|
|Mouse PDGF-C ORF mammalian expression plasmid, C-Flag tag||MG50458-CF|
|Mouse PDGF-C ORF mammalian expression plasmid, C-His tag||MG50458-CH|
|Mouse PDGF-C ORF mammalian expression plasmid, C-Myc tag||MG50458-CM|
|Mouse PDGF-C ORF mammalian expression plasmid, C-HA tag||MG50458-CY|
|Mouse PDGF-C ORF mammalian expression plasmid, N-Flag tag||MG50458-NF|
|Mouse PDGF-C ORF mammalian expression plasmid, N-His tag||MG50458-NH|
|Mouse PDGF-C ORF mammalian expression plasmid, N-Myc tag||MG50458-NM|
|Mouse PDGF-C ORF mammalian expression plasmid, N-HA tag||MG50458-NY|
|Mouse PDGF-C natural ORF mammalian expression plasmid||MG50458-UT|
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PDGF-C is a member of the PDGF/VEGF family of growth factors with a unique domain organization and expression pattern. Platelet-derived growth factor receptors (PDGFRs) are catalytic receptors that have intracellular tyrosine kinase activity. They have roles in the regulation of many biological processes including embryonic development, angiogenesis, cell proliferation and differentiation, and contribute to the pathophysiology of some diseases, including cancer. There are two isoforms of the PDGFR receptor; PDGFRalpha and PDGFRbeta, which can form homo- or heterodimers. The endogenous PDGFR ligands are PDGF-A, -B, -C and -D, which induce receptor dimerization and transphosphorylation at specific tyrosine residues upon binding. This activates the intracellular kinase activity, initiating intracellular signaling through the MAPK, PI 3-K and PKCgamma pathways. PDGF-C acts as a specific ligand for alpha platelet-derived growth factor receptor homodimer, and alpha and beta heterodimer. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. PDGF-C Appears to be involved in the three stages of wound healing: inflammation, proliferation and remodeling. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs.