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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse OMD ORF mammalian expression plasmid, C-GFPSpark tag||MG50451-ACG|
|Mouse OMD ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50451-ACR|
|Mouse OMD ORF mammalian expression plasmid, C-Flag tag||MG50451-CF|
|Mouse OMD ORF mammalian expression plasmid, C-His tag||MG50451-CH|
|Mouse OMD ORF mammalian expression plasmid, C-Myc tag||MG50451-CM|
|Mouse OMD ORF mammalian expression plasmid, C-HA tag||MG50451-CY|
|Mouse OMD ORF mammalian expression plasmid, N-Flag tag||MG50451-NF|
|Mouse OMD ORF mammalian expression plasmid, N-His tag||MG50451-NH|
|Mouse OMD ORF mammalian expression plasmid, N-Myc tag||MG50451-NM|
|Mouse OMD ORF mammalian expression plasmid, N-HA tag||MG50451-NY|
|Mouse OMD natural ORF mammalian expression plasmid||MG50451-UT|
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Osteomodulin (OMD), also known as Osteoadherin (OSAD), Keratan sulfate proteoglycan osteomodulin, KSPG osteomodulin, and SLRR2C, is a secreted protein which belongs to the small leucine-rich proteoglycan (SLRP) family and Class II subfamily. SLRP family proteins are normally found in extracellular matrices, but Osteomodulin is the only member restricted to mineralized tissues. Osteomodulin is primarily expressed by osteoblasts and might have a role in regulation of mineralization. In bone OSAD has been localized in primary spongiosa within the bovine fetal rib growth plate. Moreover, in situ hybridization has shown expression of OSAD in osteoblasts close to the cartilage and bone border in the growth plate of rat femur. OSAD may play an important role during tooth development and biomineralization of dentin. Osteomodulin is a cell binding keratan sulfate proteoglycan which was recently isolated from mineralized bovine bone and subsequently cloned and sequenced. Osteomodulin may be implicated in biomineralization processes. It has a function in binding of osteoblasts via the alpha (V) beta (3)-integrin. It is likely that Osteomodulin is an osteoblast maturation marker that is induced by osteoclast activity. Osteomodulin is also an early marker for terminally differentiated matrix producing osteoblasts.