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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse ACE2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50249-ACG|
|Mouse ACE2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50249-ACR|
|Mouse ACE2 ORF mammalian expression plasmid, C-Flag tag||MG50249-CF|
|Mouse ACE2 ORF mammalian expression plasmid, C-His tag||MG50249-CH|
|Mouse ACE2 ORF mammalian expression plasmid, C-Myc tag||MG50249-CM|
|Mouse ACE2 ORF mammalian expression plasmid, C-HA tag||MG50249-CY|
|Mouse ACE2 ORF mammalian expression plasmid, N-Flag tag||MG50249-NF|
|Mouse ACE2 ORF mammalian expression plasmid, N-His tag||MG50249-NH|
|Mouse ACE2 ORF mammalian expression plasmid, N-Myc tag||MG50249-NM|
|Mouse ACE2 ORF mammalian expression plasmid, N-HA tag||MG50249-NY|
|Mouse ACE2 natural ORF mammalian expression plasmid||MG50249-UT|
|Learn more about expression Vectors|
Angiotensin-converting enzyme 2 (ACE2), a first homolog of ACE, regulates the renin angiotensin system (RAS) by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular, renal and respiratory systems. ACE2 also has an important role in blood pressure control. This enzyme, an homolog of ACE, hydrolyzes angiotensin (Ang) I to produce Ang-(1-9), which is subsequently converted into Ang-(1-7) by a neutral endopeptidase and ACE. ACE2 releases Ang-(1-7) more efficiently than its catalysis of Ang-(1-9) by cleavage of Pro(7)-Phe(8) bound in Ang II. Thus, the major biologically active product of ACE2 is Ang-(1-7), which is considered to be a beneficial peptide of the RAS cascade in the cardiovascular system. A physiological role for ACE2 has been implicated in hypertension, cardiac function, heart function and diabetes, and as a receptor of the severe acute respiratory syndrome coronavirus. In the acute respiratory distress syndrome (ARDS), ACE, AngII, and AT1R promote the disease pathogenesis, whereas ACE2 and the AT2R protect from ARDS. Importantly, ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Furthermore, the recent explosion of research into the ACE2 homolog, collectrin, has revealed a new physiological function of ACE2 as an amino acid transporter, which explains the pathogenic role of gene mutations in Hartnup disorder. This review summarizes and discusses the recently unveiled roles for ACE2 in disease pathogenesis.