|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CLEC7A ORF mammalian expression plasmid, C-GFPSpark tag||MG50233-ACG|
|Mouse CLEC7A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50233-ACR|
|Mouse CLEC7A ORF mammalian expression plasmid, C-Flag tag||MG50233-CF|
|Mouse CLEC7A ORF mammalian expression plasmid, C-His tag||MG50233-CH|
|Mouse CLEC7A ORF mammalian expression plasmid, C-Myc tag||MG50233-CM|
|Mouse CLEC7A ORF mammalian expression plasmid, C-HA tag||MG50233-CY|
|Mouse CLEC7A Gene cDNA clone plasmid||MG50233-M|
|Mouse CLEC7A ORF mammalian expression plasmid, N-Flag tag||MG50233-NF|
|Mouse CLEC7A ORF mammalian expression plasmid, N-His tag||MG50233-NH|
|Mouse CLEC7A ORF mammalian expression plasmid, N-Myc tag||MG50233-NM|
|Mouse CLEC7A ORF mammalian expression plasmid, N-HA tag||MG50233-NY|
|Mouse CLEC7A natural ORF mammalian expression plasmid||MG50233-UT|
|Learn more about expression Vectors|
Dectin-1 was recently identified as the most important receptor for beta-glucan. It is a type II transmembrane protein which binds beta-1,3 and beta-1,6 glucans, and is expressed on most cells of the innate immune system and has been implicated in phagocytosis as well as killing of fungi by macrophages, neutrophils and dendritic cells. Recognition of beta-glucan by dectin-1 triggers effective immune response, including phagocytosis and proinflammatory factor production, to eliminate infecting fungi, which especially benefits immunocompromised patients against opportunistic fungal infection. In addition, dectin-1 is involved in the adaptive immune response as well as autoimmune diseases and immune tolerance. Dectin-1 can recognize and respond to live fungal pathogens and is being increasingly appreciated as having a key role in the innate responses to these pathogens. In addition to its exogenous ligands, Dectin-1 can recognize an unidentified endogenous ligand on T cells and may act as a co-stimulatory molecule. Recent studies have highlighted the importance of Dectin-1 in anti-fungal immunity, in both mice and humans, and have suggested a possible involvement of this receptor in the control of mycobacterial infections.