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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse S100A8 ORF mammalian expression plasmid, C-GFPSpark tag||MG50228-ACG|
|Mouse S100A8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50228-ACR|
|Mouse S100A8 ORF mammalian expression plasmid, C-Flag tag||MG50228-CF|
|Mouse S100A8 ORF mammalian expression plasmid, C-His tag||MG50228-CH|
|Mouse S100A8 ORF mammalian expression plasmid, C-Myc tag||MG50228-CM|
|Mouse S100A8 ORF mammalian expression plasmid, C-HA tag||MG50228-CY|
|Mouse S100A8 ORF mammalian expression plasmid, N-Flag tag||MG50228-NF|
|Mouse S100A8 ORF mammalian expression plasmid, N-His tag||MG50228-NH|
|Mouse S100A8 ORF mammalian expression plasmid, N-Myc tag||MG50228-NM|
|Mouse S100A8 ORF mammalian expression plasmid, N-HA tag||MG50228-NY|
|Mouse S100A8 natural ORF mammalian expression plasmid||MG50228-UT|
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S100A8 is a member of the S100 protein family containing 2EF-hand calcium-binding motifs. S100 proteins are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of S100A8 protein is associated with various diseases and cancers. S100A8 may have an immunoregulatory role by contributing to the regulation of fetal-maternal interactions. It may play a protective role and its absence may allow infiltration by maternal cells, a process eventually manifesting as resorption. The heterodimeric S100 protein complex S100A8/A9 which has been shown to be involved in inflammatory and neoplastic disorders. The complex can induce cell proliferation, or apoptosis, inflammation, collagen synthesis, and cell migration. S100A8/A9 has emerged as important pro-inflammatory mediator in acute and chronic inflammation. More recently, increased S100A8 and S100A9 levels were also detected in various human cancers, presenting abundant expression in neoplastic tumor cells as well as infiltrating immune cells. On the one hand, S100A8/A9 is a powerful apoptotic agent produced by immune cells, making it a very fascinating tool in the battle against cancer. It spears the risk to induce auto-immune response and may serve as a lead compound for cancer-selective therapeutics. In contrast, S100A8/A9 expression in cancer cells has also been associated with tumor development, cancer invasion or metastasis. Altogether, its expression and potential cytokine-like function in inflammation and in cancer suggests that S100A8/A9 may play a key role in inflammation-associated cancer.