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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse FGFR4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50194-ACG|
|Mouse FGFR4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50194-ACR|
|Mouse FGFR4 ORF mammalian expression plasmid, C-Flag tag||MG50194-CF|
|Mouse FGFR4 ORF mammalian expression plasmid, C-His tag||MG50194-CH|
|Mouse FGFR4 ORF mammalian expression plasmid, C-Myc tag||MG50194-CM|
|Mouse FGFR4 ORF mammalian expression plasmid, C-HA tag||MG50194-CY|
|Mouse FGFR4 ORF mammalian expression plasmid, N-Flag tag||MG50194-NF|
|Mouse FGFR4 ORF mammalian expression plasmid, N-His tag||MG50194-NH|
|Mouse FGFR4 ORF mammalian expression plasmid, N-Myc tag||MG50194-NM|
|Mouse FGFR4 ORF mammalian expression plasmid, N-HA tag||MG50194-NY|
|Mouse FGFR4 natural ORF mammalian expression plasmid||MG50194-UT|
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Fibroblast growth factor receptor 4 (FGFR4) also known as CD334 antigen or tyrosine kinase related to fibroblast growth factor receptor, is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR4/CD334 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR4/CD334 preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. FGFR4/CD334 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4/CD334. Mutations in FGFR4/CD334 lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.