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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse TREM2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50149-ACG|
|Mouse TREM2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50149-ACR|
|Mouse TREM2 ORF mammalian expression plasmid, C-Flag tag||MG50149-CF|
|Mouse TREM2 ORF mammalian expression plasmid, C-His tag||MG50149-CH|
|Mouse TREM2 ORF mammalian expression plasmid, C-Myc tag||MG50149-CM|
|Mouse TREM2 ORF mammalian expression plasmid, C-HA tag||MG50149-CY|
|Mouse TREM2 ORF mammalian expression plasmid, N-Flag tag||MG50149-NF|
|Mouse TREM2 ORF mammalian expression plasmid, N-His tag||MG50149-NH|
|Mouse TREM2 ORF mammalian expression plasmid, N-Myc tag||MG50149-NM|
|Mouse TREM2 ORF mammalian expression plasmid, N-HA tag||MG50149-NY|
|Mouse TREM2 natural ORF mammalian expression plasmid||MG50149-UT|
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Triggering receptor expressed on myeloid cells 2 ( TREM2 ) is a single Ig domain receptor. It is expressed on macrophages and dendritic cells but not on granulocytes or monocytes. Its expression is most abundant in the basal ganglia, corpus callosum, medulla oblongata and spinal cord, and microglial cells are the major TREM2-producing cell type in the central nervous system (CNS). TREM2 may play a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM2 forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. It also associates with the signal adapter protein, DAP12, which has a cytoplasmic ITAM, leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is both required and sufficient for competent uptake of apoptotic neuronal cells. TREM2 and TREM2-L form a receptor-ligand pair connecting microglia with apoptotic neurons, directing removal of damaged cells to allow repair. Deficiency of the adapter protein DAP12 or its associated receptor TREM2 is associated with abnormal osteoclast development in humans. Defects in TREM2 are causes of PLOSL, also known as NHD. In addition, TREM2 signaling is also an important pathway to promote healing of wounds in the colon where stem cell replacement is necessary.