|Datasheet||Specific References||Reviews||Related Products||Protocols|
pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse OSM ORF mammalian expression plasmid, C-GFPSpark tag||MG50112-ACG|
|Mouse OSM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50112-ACR|
|Mouse OSM ORF mammalian expression plasmid, C-Flag tag||MG50112-CF|
|Mouse OSM ORF mammalian expression plasmid, C-His tag||MG50112-CH|
|Mouse OSM ORF mammalian expression plasmid, C-Myc tag||MG50112-CM|
|Mouse OSM ORF mammalian expression plasmid, C-HA tag||MG50112-CY|
|Mouse OSM ORF mammalian expression plasmid, N-Flag tag||MG50112-NF|
|Mouse OSM ORF mammalian expression plasmid, N-His tag||MG50112-NH|
|Mouse OSM ORF mammalian expression plasmid, N-Myc tag||MG50112-NM|
|Mouse OSM ORF mammalian expression plasmid, N-HA tag||MG50112-NY|
|Mouse OSM natural ORF mammalian expression plasmid||MG50112-UT|
|Learn more about expression Vectors|
Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 family of cytokines that has functions mainly in cell growth. Oncostatin M (OSM) is considered as a pleiotropic cytokine that signals through cell surface receptors typeⅠand typeⅡ both of which share the similarity of containing protein gp130 and takes part in many biometabolism processes including liver development, haematopoeisis, inflammation, bone formation and destruction and possibly CNS development. Oncostatin M (OSM) was previoustly identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It also has been reported that OSM, like LIF, IL-6 and G-CSF, has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells. The human form of OSM is insensitive between pH2 and 11 and resistant to heating for one hour at 56 degree but is not stable at 90 degrees. The human OSM is produced as a precursor containing 252 amino acids, whose first 25 amino acids function as a secretory signal peptide and which on removal yields the soluble 227 amino acid pro-OSM. Removal of the C-teminal most 31 amino acids produces the fully active 196 residue form.