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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse IGF-II ORF mammalian expression plasmid, C-GFPSpark tag||MG50040-ACG|
|Mouse IGF-II ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50040-ACR|
|Mouse IGF-II ORF mammalian expression plasmid, C-Flag tag||MG50040-CF|
|Mouse IGF-II ORF mammalian expression plasmid, C-His tag||MG50040-CH|
|Mouse IGF-II ORF mammalian expression plasmid, C-Myc tag||MG50040-CM|
|Mouse IGF-II ORF mammalian expression plasmid, C-HA tag||MG50040-CY|
|Mouse IGF-II ORF mammalian expression plasmid, N-Flag tag||MG50040-NF|
|Mouse IGF-II ORF mammalian expression plasmid, N-His tag||MG50040-NH|
|Mouse IGF-II ORF mammalian expression plasmid, N-Myc tag||MG50040-NM|
|Mouse IGF-II ORF mammalian expression plasmid, N-HA tag||MG50040-NY|
|Mouse IGF-II natural ORF mammalian expression plasmid||MG50040-UT|
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Insulin-like growth factor 2 (IGF-2/IGF-II) is a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. IGF-2/IGF-II is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland. IGF-2 and exhibited statistically significant, positive associations with colorectal cancer risk when cases were confined to those diagnosed within a relatively short time period after enrolment. Circulating IGF-2 and IGFBP-3 can serve as early indicators of impending colorectal cancer. IGF-2/IGF-II appears to be involved in the progression of many tumours. It binds to at least two different types of receptor: IGF type 1 (IGF 1R) and mannose 6-phosphate/IGF type 2 (M6-P/IGF 2R). Ligand binding to IGF 1R provokes mitogenic and anti-apoptotic effects. M6-P/IGF 2R has a tumour suppressor function—it mediates IGF 2 degradation. Mutation of M6-P/IGF 2R causes both diminished growth suppression and augmented growth stimulation. The aim of this study was to investigate the role of IGF 2 and its receptors (IGF 1R and IGF 2R) in human gastric cancer.