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Mouse Frizzled-2 Gene cDNA Clone (full-length ORF Clone)

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Frizzled-2/FZD2cDNA Clone Product Information
Gene_bank_ref_id:NM_020510.2
cDNA Size:1713
cDNA Description:ORF Clone of Mus musculus frizzled homolog 2 (Drosophila) DNA.
Gene Synonym:Fz10, Fzd10, Mfz10, Mfz10a, AL033370, AW456835
Species:Mouse
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for ten point mutations: 9C/T, 12C/T, 55C/T, 78A/G, 696C/T, 775 T/C and 792 T/C not causing the amino acid variation, 577 T/G resulting in the amino acid 193Ser substitution by Ala, 1636 T/G resulting in the amino acid 546Ser substitution by Ala and 1711-1713 TGA/AAT resulting in the amino acid terminator substitution by Asn.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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Catalog:MG50032-M
List Price: $115.00  (Save $0.00)
Price:$115.00      [How to order]
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