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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse sFRP-2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50028-ACG|
|Mouse sFRP-2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50028-ACR|
|Mouse sFRP-2 ORF mammalian expression plasmid, C-Flag tag||MG50028-CF|
|Mouse sFRP-2 ORF mammalian expression plasmid, C-His tag||MG50028-CH|
|Mouse sFRP-2 ORF mammalian expression plasmid, C-Myc tag||MG50028-CM|
|Mouse sFRP-2 ORF mammalian expression plasmid, C-HA tag||MG50028-CY|
|Mouse sFRP-2 ORF mammalian expression plasmid, N-Flag tag||MG50028-NF|
|Mouse sFRP-2 ORF mammalian expression plasmid, N-His tag||MG50028-NH|
|Mouse sFRP-2 ORF mammalian expression plasmid, N-Myc tag||MG50028-NM|
|Mouse sFRP-2 ORF mammalian expression plasmid, N-HA tag||MG50028-NY|
|Mouse sFRP-2 natural ORF mammalian expression plasmid||MG50028-UT|
|Learn more about expression Vectors|
The Secreted frizzled-related protein (SFRP) family consists of five secreted glycoproteins in humans (SFRP1~5) that act as extracellular signaling ligands. Each SFRP is approximately 300 amino acids in length and contains a cysteine-rich domain (CRD) that shares 30-50% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. sFRP2 is a member of the SFRP family acting as soluble modulators of Wnt signaling and contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins called FZ domain and a NTR domain.sFRP2 inhibites hypoxia induced endothelial cell apoptosis and increases endothelial cell migration. It prevents mesoderm specification and maintains the cells in the undifferentiated state. SFRP2 is also a novel stimulator of angiogenesis that stimulates angiogenesis via a calcineurin/NFAT pathway, thus is regarded as a favorable target for the inhibition of angiogenesis in solid tumors. Mouse sFRP2 is highly expressed in the eye and is also detected in heart and lung at low level.