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Human PAX8 Gene cDNA clone plasmid

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Human PAX8 cDNA Clone Product Information
Gene_bank_ref_id:BC001060
RefSeq ORF Size:1353bp
cDNA Description:Full length Clone DNA of Homo sapiens paired box 8.
Gene Synonym:PAX8
Species:Human
Vector:pGEM-T Vector
Plasmid:pGEM-PAX8
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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Background

PAX8 gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins which contain a paired box domain, an octapeptide, and a paired-type homeodomain. PAX8 is involved in thyroid follicular cell development and expression of thyroid-specific genes. Also functions in very early stages of kidney organogenesis. Mutations in PAX8 gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

References
  • Poleev A. et al., 1995, Eur J Biochem. 228 (3): 899-911
  • Poleev A. et al., 1993, Development. 116 (3): 611-23.
  • Di Palma. et al., 2003, J Biol Chem. 278 (5): 3395-402.
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    Catalog: HG14505-G
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