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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human TIMD4 ORF mammalian expression plasmid, C-GFPSpark tag||HG12161-ACG|
|Human TIMD4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12161-ACR|
|Human TIMD4 ORF mammalian expression plasmid, C-Flag tag||HG12161-CF|
|Human TIMD4 ORF mammalian expression plasmid, C-His tag||HG12161-CH|
|Human TIMD4 ORF mammalian expression plasmid, C-Myc tag||HG12161-CM|
|Human TIMD4 ORF mammalian expression plasmid, C-HA tag||HG12161-CY|
|Human TIMD4 ORF mammalian expression plasmid, N-Flag tag||HG12161-NF|
|Human TIMD4 ORF mammalian expression plasmid, N-His tag||HG12161-NH|
|Human TIMD4 ORF mammalian expression plasmid, N-Myc tag||HG12161-NM|
|Human TIMD4 ORF mammalian expression plasmid, N-HA tag||HG12161-NY|
|Human TIMD4 natural ORF mammalian expression plasmid||HG12161-UT|
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A type I transmembrane protein called TIM4 (T-cell immunoglobulin- and mucin-domain-containing molecule; also known as TIMD4), which belongs to the immunoglobulin superfamily and TIM family. TIM4 is involved in regulating T-cell proliferation and lymphotoxin signaling. It is a ligand for HAVCR1/TIMD1. Recent reports indicate that dendritic cell (DC)-derived T-cell immunoglobulin and mucin domain molecule (TIM)-4, which is expressed on dendritic cells and macrophages, plays an important role in the initiation of T(H)2 polarization. TIM4 bound apoptotic cells by recognizing phosphatidylserine via its immunoglobulin domain. The expression of TIM4 in fibroblasts enhanced their ability to engulf apoptotic cells. TIM4 is phosphatidylserine receptor for the engulfment of apoptotic cells, and may also be involved in intercellular signalling in which exosomes are involved. Modulation of TIM4 production in dendritic cells (DCs) represents a novel therapeutic approach for the treatment of peanut allergy. The interaction of TIM1/TIM4 played a critical role in sustaining the polarization status of Th2 cells in allergic rhinitis (AR) patients. Cross-linking FcgammaRI by antigen/IgG complexes increased the production of TIM4 by dendritic cells via upregulating tumor necrosis factor-alpha in DCs. Specific immunotherapy (SIT) suppresses the skewed Th2 responses via disrupting the interaction of TIM1/TIM4 in antigen-specific Th2 cells.