|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human EGFL7 ORF mammalian expression plasmid, C-GFPSpark tag||HG11979-ACG|
|Human EGFL7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11979-ACR|
|Human EGFL7 ORF mammalian expression plasmid, C-Flag tag||HG11979-CF|
|Human EGFL7 ORF mammalian expression plasmid, C-His tag||HG11979-CH|
|Human EGFL7 ORF mammalian expression plasmid, C-Myc tag||HG11979-CM|
|Human EGFL7 ORF mammalian expression plasmid, C-HA tag||HG11979-CY|
|Human EGFL7 Gene cDNA clone plasmid||HG11979-G|
|Human EGFL7 ORF mammalian expression plasmid, N-Flag tag||HG11979-NF|
|Human EGFL7 ORF mammalian expression plasmid, N-His tag||HG11979-NH|
|Human EGFL7 ORF mammalian expression plasmid, N-Myc tag||HG11979-NM|
|Human EGFL7 ORF mammalian expression plasmid, N-HA tag||HG11979-NY|
|Human EGFL7 natural ORF mammalian expression plasmid||HG11979-UT|
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Epidermal growth factor-like protein 7, also known as EGF-like protein 7, Multiple epidermal growth factor-like domains protein 7, Multiple EGF-like domains protein 7, Vascular endothelial statin, NOTCH4-like protein and EGFL7, is a secreted protein which contains two EGF-like domains and one EMI domain. EGFL7 was identified by a number of groups as a putative secreted factor produced by the vascular endothelial cells (ECs). EGFL7 is described as a novel endothelial cell-derived factor involved in the regulation of the spatial arrangement of cells during vascular tube assembly. With its impact on tubulogenesis and vessel shape EGFL7 joined the large family of molecules governing blood vessel formation. EGFL7 regulates midline angioblast migration in zebrafish embryos-a key step in vascular tubulogenesis. EGFL7 is tightly associated with the extracellular matrix (ECM), and it supports EC migration either as a single factor or in combination with other ECM molecules. EGFL7 provides a proper microenvironment for endothelial cell migration, thereby enabling accurate patterning. Our study indicates that the molecular composition of the ECM influences vascular morphogenesis. EGFL7 also regulates vascular tubulogenesis. It inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate.