|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human VNN2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11728-ACG|
|Human VNN2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11728-ACR|
|Human VNN2 ORF mammalian expression plasmid, C-Flag tag||HG11728-CF|
|Human VNN2 ORF mammalian expression plasmid, C-His tag||HG11728-CH|
|Human VNN2 ORF mammalian expression plasmid, C-Myc tag||HG11728-CM|
|Human VNN2 ORF mammalian expression plasmid, C-HA tag||HG11728-CY|
|Human VNN2 Gene cDNA clone plasmid||HG11728-G|
|Human VNN2 ORF mammalian expression plasmid, N-Flag tag||HG11728-NF|
|Human VNN2 ORF mammalian expression plasmid, N-His tag||HG11728-NH|
|Human VNN2 ORF mammalian expression plasmid, N-Myc tag||HG11728-NM|
|Human VNN2 ORF mammalian expression plasmid, N-HA tag||HG11728-NY|
|Human VNN2 natural ORF mammalian expression plasmid||HG11728-UT|
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Vascular non-inflammatory molecule 2, also known as glycosyl-phosphatidyl inositol-anchored protein GPI-80, Vanin-2, Protein FOAP-4 and VNN2, is a cell membrane protein which belongs to the CN hydrolase family and Vanin subfamily. VNN2 is widely expressed with higher expression in spleen and blood. VNN2 is a member of the vanin family of proteins which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. VNN2 is an amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. It is involved in the thymus homing of bone marrow cells. VNN2 plays a role in transendothelial migration of neutrophils and may regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil.