|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IL12RB1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11674-ACG|
|Human IL12RB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11674-ACR|
|Human IL12RB1 ORF mammalian expression plasmid, C-Flag tag||HG11674-CF|
|Human IL12RB1 ORF mammalian expression plasmid, C-His tag||HG11674-CH|
|Human IL12RB1 ORF mammalian expression plasmid, C-Myc tag||HG11674-CM|
|Human IL12RB1 ORF mammalian expression plasmid, C-HA tag||HG11674-CY|
|Human IL12RB1 Gene cDNA clone plasmid||HG11674-M|
|Human IL12RB1 natural ORF mammalian expression plasmid||HG11674-M-N|
|Human IL12RB1 ORF mammalian expression plasmid, N-Flag tag||HG11674-NF|
|Human IL12RB1 ORF mammalian expression plasmid, N-His tag||HG11674-NH|
|Human IL12RB1 ORF mammalian expression plasmid, N-Myc tag||HG11674-NM|
|Human IL12RB1 ORF mammalian expression plasmid, N-HA tag||HG11674-NY|
|Human IL12RB1 natural ORF mammalian expression plasmid||HG11674-UT|
|Learn more about expression Vectors|
Interleukin 12 receptor, beta 1 is also known as IL-12 receptor beta component, IL-12R subunit beta-1, and CD212 antigen (CD212). IL12RB1(CD212) is a subunit of the interleukin 12 receptor. IL12RB1(CD212) is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. IL12RB1(CD212) forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of IL12RB1 and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The lack of expression of this gene was found to result in the immunodeficiency of patients with severe mycobacterial and Salmonella infections. IL12RB1(CD212) Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. It associated with IL12RB2 it forms a functional, high affinity receptor for IL12. IL12RB1(CD212) associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.