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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human VNN1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11662-ACG|
|Human VNN1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11662-ACR|
|Human VNN1 ORF mammalian expression plasmid, C-Flag tag||HG11662-CF|
|Human VNN1 ORF mammalian expression plasmid, C-His tag||HG11662-CH|
|Human VNN1 ORF mammalian expression plasmid, C-Myc tag||HG11662-CM|
|Human VNN1 ORF mammalian expression plasmid, C-HA tag||HG11662-CY|
|Human VNN1 ORF mammalian expression plasmid, N-Flag tag||HG11662-NF|
|Human VNN1 ORF mammalian expression plasmid, N-His tag||HG11662-NH|
|Human VNN1 ORF mammalian expression plasmid, N-Myc tag||HG11662-NM|
|Human VNN1 ORF mammalian expression plasmid, N-HA tag||HG11662-NY|
|Human VNN1 natural ORF mammalian expression plasmid||HG11662-UT|
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Pantetheinase, also known as Pantetheine hydrolase, Vascular non-inflammatory molecule 1, Vanin-1, and VNN1, is a cell membrane protein which belongs to the CN hydrolase family and BTD/VNN subfamily. Vanin-1 contains one CN hydrolase domain. It is widely expressed with higher expression in spleen, kidney and blood. It is overexpressed in lesional psoriatic skin. Vanin-1 is also a member of the Vanin family of proteins which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. Vanin-1 is an epithelial pantetheinase that provides cysteamine to tissue and regulates response to stress. Vanin-1 is expressed by enterocytes, and its absence limits intestinal epithelial cell production of proinflammatory signals. Vanin-1 regulates late adhesion steps of thymus homing under physiological, noninflammatory conditions. The early impact of vanin-1 deficiency on tumor induction was directly correlated to the amount of inflammation and subsequent epithelial proliferation rather than cell death rate. Vanin-1 molecule was shown to be involved in the control of thymus reconstitution following sublethal irradiation.