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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human EPHA7 ORF mammalian expression plasmid, C-GFPSpark tag||HG11657-ACG|
|Human EPHA7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11657-ACR|
|Human EPHA7 ORF mammalian expression plasmid, C-Flag tag||HG11657-CF|
|Human EPHA7 ORF mammalian expression plasmid, C-His tag||HG11657-CH|
|Human EPHA7 ORF mammalian expression plasmid, C-Myc tag||HG11657-CM|
|Human EPHA7 ORF mammalian expression plasmid, C-HA tag||HG11657-CY|
|Human EPHA7 ORF mammalian expression plasmid, N-Flag tag||HG11657-NF|
|Human EPHA7 ORF mammalian expression plasmid, N-His tag||HG11657-NH|
|Human EPHA7 ORF mammalian expression plasmid, N-Myc tag||HG11657-NM|
|Human EPHA7 ORF mammalian expression plasmid, N-HA tag||HG11657-NY|
|Human EPHA7 natural ORF mammalian expression plasmid||HG11657-UT|
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Ephrin type-A receptor 7, also known as EphA7, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Eph receptor-mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. Down-regulation of EphA7 secondary to hypermethylation has been reported in colorectal cancer. The expression of EphA7 was reduced in all tested gastric cancer cell lines; however, there is marked variability in expression among gastric carcinoma specimens. EphA7 may have roles in the pathogenesis and development of gastric carcinomas.