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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human ADM ORF mammalian expression plasmid, C-GFPSpark tag||HG11646-ACG|
|Human ADM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11646-ACR|
|Human ADM ORF mammalian expression plasmid, C-Flag tag||HG11646-CF|
|Human ADM ORF mammalian expression plasmid, C-His tag||HG11646-CH|
|Human ADM ORF mammalian expression plasmid, C-Myc tag||HG11646-CM|
|Human ADM ORF mammalian expression plasmid, C-HA tag||HG11646-CY|
|Human ADM ORF mammalian expression plasmid, N-Flag tag||HG11646-NF|
|Human ADM ORF mammalian expression plasmid, N-His tag||HG11646-NH|
|Human ADM ORF mammalian expression plasmid, N-Myc tag||HG11646-NM|
|Human ADM ORF mammalian expression plasmid, N-HA tag||HG11646-NY|
|Human ADM natural ORF mammalian expression plasmid||HG11646-UT|
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Adrenomedullin consists of 52 amino acids and is a member of the adrenomedullin family. It s a a hypotensive peptide and has 1 intramolecular disulfide bond. It seems that adrenomedullin has a slight homology with the calcitonin gene-related peptide. Adrenomedullin has a highly expression in pheochromocytoma and adrenal medulla. It also can be detected in lung, ventricle and kidney tissues. Adrenomedullin and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, adrenomedullin is diuretic and natriuretic, and both adrenomedullin and PAMP inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels. It is believed that adrenomedullin functions through combinations of the calcitonin receptor like receptor and receptor activity-modifying proteins complexes, as well as CGRP receptors.