|Datasheet||Specific References||Reviews||Related Products||Protocols|
pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human REG3G ORF mammalian expression plasmid, C-GFPSpark tag||HG11641-ACG|
|Human REG3G ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11641-ACR|
|Human REG3G ORF mammalian expression plasmid, C-Flag tag||HG11641-CF|
|Human REG3G ORF mammalian expression plasmid, C-His tag||HG11641-CH|
|Human REG3G ORF mammalian expression plasmid, C-Myc tag||HG11641-CM|
|Human REG3G ORF mammalian expression plasmid, C-HA tag||HG11641-CY|
|Human REG3G ORF mammalian expression plasmid, N-Flag tag||HG11641-NF|
|Human REG3G ORF mammalian expression plasmid, N-His tag||HG11641-NH|
|Human REG3G ORF mammalian expression plasmid, N-Myc tag||HG11641-NM|
|Human REG3G ORF mammalian expression plasmid, N-HA tag||HG11641-NY|
|Human REG3G natural ORF mammalian expression plasmid||HG11641-UT|
|Learn more about expression Vectors|
Regenerating gene (Reg), first isolated from a regenerating islet cDNA library, encodes a secretory protein with a growth stimulating effect on pancreatic beta cells. Reg and Reg-related genes which were expressed in various organs have been revealed to constitute a multigene family, the Reg family, which consists of four subtypes (types I, II, III, IV) based on the primary structures of the encoded proteins of the genes, which are associated with tissue repair and have been directly implicated in pancreatic beta-cell regeneration. Reg proteins are expressed in various organs and are involved in cancers and neurodegenerative diseases. They display a typical C-type lectin-like domain but possess additional highly conserved amino acids. Regenerating islet-derived 3 gamma (REG3G), also known as pancreatitis-associated protein 1B (PAP1B), is a member of the secreted Reg superfamily and contains one typical C-type lectin domain. REG3G is expressed weakly in pancreas, strongly in intestinal tract, but not in hyperplastic islets REG3G might be a stress protein involved in the control of bacterial proliferation. It was indicated that REG3G specifically targets Gram-positive bacteria because it binds to their surface peptidoglycan layer, and serves as one of several antimicrobial peptides produced by paneth cells via stimulation of toll-like receptors (TLRs) by pathogen-associated molecular patterns (PAMPs).