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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human LUM ORF mammalian expression plasmid, C-GFPSpark tag||HG11640-ACG|
|Human LUM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11640-ACR|
|Human LUM ORF mammalian expression plasmid, C-Flag tag||HG11640-CF|
|Human LUM ORF mammalian expression plasmid, C-His tag||HG11640-CH|
|Human LUM ORF mammalian expression plasmid, C-Myc tag||HG11640-CM|
|Human LUM ORF mammalian expression plasmid, C-HA tag||HG11640-CY|
|Human LUM ORF mammalian expression plasmid, N-Flag tag||HG11640-NF|
|Human LUM ORF mammalian expression plasmid, N-His tag||HG11640-NH|
|Human LUM ORF mammalian expression plasmid, N-Myc tag||HG11640-NM|
|Human LUM ORF mammalian expression plasmid, N-HA tag||HG11640-NY|
|Human LUM natural ORF mammalian expression plasmid||HG11640-UT|
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Lumican, a prototypic leucine-rich proteoglycan with keratan sulfate side chains, is a major component of the cornea, dermal, and muscle connective tissues. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and is also present in the connective tissues of other organs and embryonic corneal stroma as a glycoprotein. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. lumican expressed in injured epithelium may modulate cell behavior such as adhesion or migration, thus contributing to corneal epithelial wound healing. Lumican plays a crucial role in the regulation of collagen assembly into fibrils in various connective tissues and serve as a definitive link between a necessity for lumican in the development of a highly organized collagenous matrix and corneal transparency.