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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human SIRPA ORF mammalian expression plasmid, C-GFPSpark tag||HG11612-ACG|
|Human SIRPA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11612-ACR|
|Human SIRPA ORF mammalian expression plasmid, C-Flag tag||HG11612-CF|
|Human SIRPA ORF mammalian expression plasmid, C-His tag||HG11612-CH|
|Human SIRPA ORF mammalian expression plasmid, C-Myc tag||HG11612-CM|
|Human SIRPA ORF mammalian expression plasmid, C-HA tag||HG11612-CY|
|Human SIRPA ORF mammalian expression plasmid, N-Flag tag||HG11612-NF|
|Human SIRPA ORF mammalian expression plasmid, N-His tag||HG11612-NH|
|Human SIRPA ORF mammalian expression plasmid, N-Myc tag||HG11612-NM|
|Human SIRPA ORF mammalian expression plasmid, N-HA tag||HG11612-NY|
|Human SIRPA natural ORF mammalian expression plasmid||HG11612-UT|
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Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.