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Human ENO1 Gene cDNA clone plasmid

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Human ENO1 cDNA Clone Product Information
Gene_bank_ref_id:NM_001428.2
RefSeq ORF Size:1305bp
cDNA Description:Full length Clone DNA of Homo sapiens enolase 1, (alpha).
Gene Synonym:NNE, PPH, MPB1, MBP-1, ENO1L1, ENO1
Species:Human
Vector:pMD18-T Vector
Plasmid:pMD-ENO1
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutation 1296G/A and 1302G/A not causing the amino acid variation.
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pMD18-T Vector Information

pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which multiple cloning sites as shown below. The pMD18-T Vector is 2.6kb in size and contains the amplicin resistance gene for selection. The coding sequence was inserted by TA cloning at site 425.

pMD18-T vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

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References
  • Capello M, et al. (2011) a-Enolase: a promising therapeutic and diagnostic tumor target. FEBS J. 278(7): 1064-74.
  • Kang HJ, et al. (2008) Structure of human alpha-enolase (hENO1), a multifunctional glycolytic enzyme. Acta Crystallogr D Biol Crystallogr. 64(Pt 6): 651-7.
  • Lopez-Alemany R, et al. (2005) Alpha-enolase plasminogen receptor in myogenesis. Front Biosci. 10: 30-6.
  • Ejeskdr K, et al. (2005) Introduction of in vitro transcribed ENO1 mRNA into neuroblastoma cells induces cell death. BMC Cancer. 5: 161.
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    Catalog: HG11554-M
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